Retroviruses infect a wide range of mammals and cause various disease processes in them including immunodeficiencies, neurological disorders, and tumor development. Thus, our findings support the use of BLV ECs in the field. With its ease of use and reliable detection, this new method strengthens the laboratory typing for DRB3*009:02-carrying cattle. We determined that the percentage of DRB3*009:02-carrying cattle in Kyushu Island was 10.56%. The DRB3*009:02-TaqMan assay showed high-discriminative sensitivity and specificity toward DRB3*009:02, making it suitable for identifying DRB3*009:02-carrying cattle in post-screening tests on individuals. Its capacity is sufficient for herd-level screening for DRB3*009:02-carrying cattle. Our pooled testing system detected cattle carrying the DRB3*009:02 allele from a DNA pool containing one DRB3*009:02-positive animal and 29 cattle with other alleles. Using this system, we determined the percentage of DRB3*009:02-carrying cattle on Kyushu Island, Japan. It employs a highly sensitive, specific real-time PCR assay and TaqMan MGB probes ( DRB3*009:02-TaqMan assay). To identify cattle carrying DRB3*009:02 in large populations more easily, we developed a pooled testing system. BLV ECs can act as transmission barriers when placed between uninfected and infected cattle in a barn. Most of cattle carrying this allele maintain undetectable BLV proviral loads and do not shed virus even when infected. Cattle carrying the bovine leukocyte antigen ( BoLA) -DRB3*009:02 allele ( DRB3*009:02) have a strong possibility of being BLV ECs. In the livestock production field, identifying the ECs of bovine leukemia virus (BLV) infection in cattle is desired to stop BLV transmission chains on farms. ![]() As genetically resistant individuals, the “elite controllers” (ECs) of human immunodeficiency virus infection have been focused on as the keys to developing further functional treatments in medicine.
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